Application of nitric oxide and carbon monoxide in a model of renal preservation

Abstract

Abstract Background Nitric oxide and carbon monoxide exert vasodilatory effects that minimize ischaemia–reperfusion injury. An isolated porcine kidney model was used to assess the effects of administering the nitric oxide donor sodium nitroprusside (SNP) and carbon monoxide-releasing molecule (CORM) 3 during a period of warm preservation followed by reperfusion. Methods Kidneys were perfused under warm preservation conditions after 10 min of warm ischaemia and 16 h of cold storage in four groups: SNP, control, CORM-3 and inactive CORM-3 (inactive control). Renal function and viability were assessed. Results SNP and CORM-3 increased renal blood flow (RBF) during warm preservation (P = 0·014). After reperfusion, RBF was significantly improved in the CORM-3 group compared with the control group (P = 0·019). The reduction in creatinine clearance was significantly less in the CORM-3 group than in the inactive CORM-3 group (P = 0·021), and serum creatinine levels were significantly lower (P = 0·029). There was a negative correlation between RBF during warm preservation and functional parameters during reperfusion (creatinine concentration: rs = − 0·722, P < 0·001; sodium excretion: rs = − 0·912, P < 0·001). Conclusion The beneficial vasodilatory effects of CORM-3 during warm preservation improved renal function during reperfusion; SNP exerted similar, although less pronounced, effects.