Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus)

Author:

Harper Lynsey R.1ORCID,Lawson Handley Lori1,Hahn Christoph12,Boonham Neil34,Rees Helen C.5,Gough Kevin C.6,Lewis Erin3,Adams Ian P.3,Brotherton Peter7,Phillips Susanna7,Hänfling Bernd1

Affiliation:

1. School of Environmental Sciences; University of Hull; Hull UK

2. Institute of Zoology; University of Graz; Graz Styria Austria

3. Fera; Sand Hutton; York UK

4. Newcastle University; Newcastle upon Tyne UK

5. ADAS; School of Veterinary Medicine and Science; The University of Nottingham; Leicestershire UK

6. School of Veterinary Medicine and Science; The University of Nottingham; Leicestershire UK

7. Natural England; Peterborough UK

Funder

University of Hull

Publisher

Wiley

Subject

Nature and Landscape Conservation,Ecology,Ecology, Evolution, Behavior and Systematics

Reference45 articles.

1. Maximum likelihood identification of Gaussian autoregressive moving average models;Akaike;Biometrika,1973

2. Meta-barcoding of “dirt” DNA from soil reflects vertebrate biodiversity;Andersen;Molecular Ecology,2012

3. Biomonitoring of marine vertebrates in Monterey Bay using eDNA metabarcoding;Andruszkiewicz;PLoS ONE,2017

4. Twenty-five species of frogs in a liter of water: eDNA survey for exploring tropical frog diversity;Bálint;bioRxiv,2017

5. Fitting linear mixed-effects models using lme4;Bates;Journal of Statistical Software,2015

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