CuticleTrace: A toolkit for capturing cell outlines from leaf cuticle with implications for paleoecology and paleoclimatology-Reference-Cited by-同舟云学术

CuticleTrace: A toolkit for capturing cell outlines from leaf cuticle with implications for paleoecology and paleoclimatology

Published:2024-02-02 Issue: Volume: Page:
ISSN:2168-0450
Container-title:Applications in Plant Sciences
language:en
Short-container-title:Appl Plant Sci

Author:

Lloyd Benjamin A.¹²^ORCID,Barclay Richard S.³^ORCID,Dunn Regan E.⁴⁵^ORCID,Currano Ellen D.⁶^ORCID,Mohamaad Ayuni I.⁶⁷,Skersies Kymbre⁶,Punyasena Surangi W.⁸^ORCID

Affiliation:

1. Department of Earth and Space Sciences University of Washington Johnson Hall Rm‐070, Box 351310, 4000 15th Ave. NE Seattle Washington 98195‐1310 USA

2. Smithsonian Environmental Research Center Smithsonian Institution 647 Contees Wharf Rd. Edgewater Maryland 21037‐0028 USA

3. Department of Paleobiology, National Museum of Natural History Smithsonian Institution 1000 Madison Dr. NW Washington D.C. 20560 USA

4. La Brea Tar Pits and Museum Natural History Museums of Los Angeles County 5801 S. Wilshire Blvd. Los Angeles California 90036 USA

5. Department of Earth Sciences University of Southern California 3551 Trousdale Parkway Los Angeles California 90089 USA

6. Department of Botany University of Wyoming 1000 E. University Ave. Laramie Wyoming 82071 USA

7. Department of Geological Sciences University of Florida 241 Williamson Hall, P.O. Box 112120 Gainesville Florida 32611‐2120 USA

8. Department of Plant Biology University of Illinois Urbana‐Champaign 139 Morrill Hall, 505 South Goodwin Ave. Urbana Illinois 61801 USA

Abstract

AbstractPremiseLeaf epidermal cell morphology is closely tied to the evolutionary history of plants and their growth environments and is therefore of interest to many plant biologists. However, cell measurement can be time consuming and restrictive with current methods. CuticleTrace is a suite of Fiji and R‐based functions that streamlines and automates the segmentation and measurement of epidermal pavement cells across a wide range of cell morphologies and image qualities.Methods and ResultsWe evaluated CuticleTrace‐generated measurements against those from alternate automated methods and expert and undergraduate hand tracings across a taxonomically diverse 50‐image data set of variable image qualities. We observed ~93% statistical agreement between CuticleTrace and expert hand‐traced measurements, outperforming alternate methods.ConclusionsCuticleTrace is a broadly applicable, modular, and customizable tool that integrates data visualization and cell shape measurement with image segmentation, lowering the barrier to high‐throughput studies of epidermal morphology by vastly decreasing the labor investment required to generate high‐quality cell shape data sets.

Publisher

Wiley

Subject

Plant Science,Ecology, Evolution, Behavior and Systematics

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