The Pre‐ and Post‐Column Derivatization on Monosaccharide Composition Analysis, a Review

Author:

Chen Xuexia1,Wang Yinuo1,Ye Yongjun2,Yu Huali3,Wu Bin1ORCID

Affiliation:

1. Ocean College Zhejiang University Zhoushan 321000 China

2. Zhejiang Suichang Huikang Pharmaceutical Industry Co. Suichang 323000 China

3. Lishui Institute for Quality Inspection and Testing Lishui 323000 China

Abstract

AbstractPolysaccharides, as common metabolic products in organisms, play a crucial role in the growth and development of living organisms. For humans, polysaccharides represent a class of compounds with diverse applications, particularly in the medical field. Therefore, the exploration of the monosaccharide composition and structural characteristics of polysaccharides holds significant importance in understanding their biological functions. This review provides a comprehensive overview of extraction methods and hydrolysis strategies for polysaccharides. It systematically analyzes strategies and technologies for determining polysaccharide composition and discusses common derivatization reagents employed in further polysaccharide studies. Derivatization is considered a fundamental strategy for determining monosaccharides, as it not only enhances the detectability of analytes but also increases detection sensitivity, especially in liquid chromatography (LC), capillary electrophoresis (CE), and gas chromatography (GC) techniques. The review meticulously examines pre‐column and post‐column derivatization techniques for monosaccharide analysis, categorizing them based on diverse detection methodologies. It delves into the principles and distinctive features of various derivatization reagents, offering a comparative analysis of their strengths and limitations. Ultimately, the aim is to provide guidance for selecting the most suitable derivatization approach, taking into account the structural nuances, biological functions, and reaction dynamics of polysaccharides.

Funder

National Natural Science Foundation of China

Publisher

Wiley

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