Combined Metabolite Analysis and Network Pharmacology to Elucidate the Mechanisms of Therapeutic Effect of Melastoma dodecandrum Ellagitannins on Abnormal Uterine Bleeding

Author:

Lu Qiu‐Ping12,Wu Miao‐Li12,Li Hui‐Lin12,Zhou Yu12,Xian Ming‐Hua12,Huang Wei‐Zhong3,Piao Xiu‐Hong4,Ge Yue‐Wei12ORCID

Affiliation:

1. School of Chinese Materia Medica Guangdong Pharmaceutical University Guangzhou 510006 China

2. Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of National Administration of TCM Guangdong Pharmaceutical University Guangzhou 510006 China

3. Guangdong Luofushan Sinopharm Co., Ltd. Huizhou 516133 China

4. School of Life Sciences and Biopharmaceutics Guangdong Pharmaceutical University Guangzhou 510006 China

Abstract

AbstractThe abnormal uterine bleeding (AUB) is complex and usually leads to severe anemia. Melastomadodecandrum (MD) is clinically used for the treatment of metrorrhagia bleeding. The MD ellagitannins (MD‐ETs) had been evidenced being effective at hemorrhage, and exerts biological activities upon their metabolites including ellagic acid and urolithins. In this study, the blood‐permeated metabolites from theMD‐ETs were analyzed using LC‐MS approach, and 19 metabolites including ellagic acid and urolithin A derivatives were identified. Furthermore, a network pharmacology analysis including the target prediction analysis, AUB target analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to reveal the relationships between “metabolites‐targets‐pathways”, which was further verified by molecular docking analysis. The results showed that methyl ellagic acid, urolithin A and isourolithin A produced from MD‐ETs can be absorbed into the blood, and might act on the core targets of VEGFA, SRC, MTOR, EGFR and CCND1. And the hemostatic effects were exerted through PI3K‐Akt, endocrine resistance and Rap 1 signaling pathways. These results implied the potential effective constituents and action mechanism of MD‐ETs in the therapy of AUB, which will promote the application of MD‐ETs as natural agent for the treatment of gynecological bleeding diseases.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering

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