Affiliation:
1. Graduation Program of Pharmaceutical Sciences State University of Ponta Grossa Ponta Grossa PR Brazil.
2. Department of Medicine State University of Ponta Grossa Ponta Grossa PR Brazil.
3. Department of Pharmaceutical Sciences State University of Ponta Grossa Ponta Grossa PR Brazil.
4. Department of Mathematics and Statistics State University of Ponta Grossa Ponta Grossa PR Brazil.
5. Department of Structural Molecular and Genetic Biology State University of Ponta Grossa Ponta Grossa PR Brazil.
Abstract
AbstractLeonurus japonicus Houtt. is a medicinal plant popular in Brazil as “rubim”, used in local folk medicine for several applications as an anti‐inflammatory, antioxidant, analgesic, and antimicrobial phytomedicine. The traditional use for wound healing is related; however, few studies have evaluated the wound healing activity. Thus, this study aimed to analyse the popular indication of the hydroalcoholic and aqueous extracts of L. japonicus aerial parts in a rat wound healing model. The initial chemical characterization was performed using flavonoid quantification and complemented with mass spectroscopy/chemometrics analysis. The wound‘s lesion contraction and tissue regeneration (histological study stained with hematoxylin‐eosin and picrosirius) were determined. Hydroalcoholic and aqueous extracts presented high flavonoid content, and mass spectrometry analysis of the extracts demonstrated the presence of compounds with a mass between 100–650, reinforcing the presence of polyphenolic constituents. The extracts of L. japonicus improve various wound healing phases, like inflammatory modulation, wound contraction, and collagen synthesis, resulting in faster healing in rats. These effects could be related to the extracts′ polyphenolic compounds.
Subject
Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering