Affiliation:
1. College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals Moganshan Research Institute at Deqing County Zhejiang University of Technology Zhejiang University of Technology Hangzhou 310014 China
2. Center for Human Nutrition David Geffen School of Medicine University of California Los Angeles CA-90024 USA
3. Emergency and Critical Care Center Department of Emergency Medicine Zhejiang Provincial People's Hospital (Affiliated People's Hospital Hangzhou Medical College) Hangzhou 310014 Zhejiang PR China
4. Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education Key Laboratory of Pharmaceutical Engineering of Zhejiang Province Zhejiang University of Technology Hangzhou 310014 China
Abstract
AbstractRecent studies have highlighted the potential of Saccharina japonica Polysaccharides (SJPs) in alleviating high‐fat diet (HFD)‐induced obesity by regulating gut microbiota, which warrants further exploration to elucidate the underlying structure‐activity relationship. In this study, five polysaccharide fractions (Sj−T, Sj−T‐1, Sj−T‐2, Sj−T‐3, and Sj−T‐4) with different structure characteristics were prepared from S. japonica, and their effects on HFD‐induced obesity and gut microbiota composition were investigated using C57BL/6J mice. The results revealed that oral administration of Sj−T considerably suppressed HFD‐induced obesity, glucose metabolic dysfunction, and other disordered symptoms. While, Sj−T‐2, which has the lowest molecular weight, was the most effective in alleviating HFD‐induced obesity and had the second‐best effect on improving HFD‐induced impaired glucose tolerance among the five SJPs. Supplementation with SJPs significantly modulated HFD‐induced gut microbiota dysbiosis both at the phylum and species levels, such as enriching Desulfobacterota and Actinobacteriota, while suppressing the abundance of Bacteroidota. Sj−T also dramatically restored the gut microbiota composition by modulating the abundance of many crucial gut bacterial taxa, including s_Bacteroides_acidifaciens, s_Lachnospiraceae _bacterium, and g_Lachnospiraceae_NK4A136_group. Besides, SJPs also dramatically altered the function of gut microbiota, including many carbohydrate‐metabolism enzymes. This study highlights the potential of SJPs in preventing obesity and restoring intestinal homeostasis in obese individuals.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Higher Education Discipline Innovation Project