Affiliation:
1. Department of Chemistry School of Advanced Sciences Vellore Institute of Technology Vellore 632014 India
2. Department of Chemistry School of Advanced Sciences and Languages VIT Bhopal University Bhopal 466114
3. Department of Integrative Biology School of Bioscience and Technology Vellore Institute of Technology Vellore 632014
4. Department of Biotechnology School of Bioscience and Technology Vellore Institute of Technology Vellore 632014
Abstract
AbstractFlavonoids epitomize structural scaffolds in many biologically active synthetic and natural compounds. They showcase a diverse spectrum of biological activities including anticancer, antidiabetic, antituberculosis, antimalarial, and antibiofilm activities. The antibiofilm activity of a series of new chalcones and flavonols against clinically significant Pseudomonas aeruginosa PAO1 strain was studied. Antivirulence activities were screened by analysing the effect of compounds on the production of virulence factors like pyocyanin, LasA protease, cell surface hydrophobicity, and rhamnolipid. The best ligands towards the quorum sensing proteins LasR, RhlR, and PqsR were recognised using a molecular docking study. The gene expression in P. aeruginosa after treatment with test compounds was evaluated on quorum sensing genes including rhlA, lasB, and pqsE. The antibiofilm potential of chalcones and flavonols was confirmed by the efficient reduction in the production of virulence factors and downregulation of gene expression.