Chemical Composition, Anthelmintic Activity, and Mechanism of Action of <i>Lippia dominguensis</i> Mold. Essential Oil on <i>Haemonchus contortus</i>-Reference-Cited by-同舟云学术

Chemical Composition, Anthelmintic Activity, and Mechanism of Action of Lippia dominguensis Mold. Essential Oil on Haemonchus contortus

Published:2023-06-16 Issue:7 Volume:20 Page:
ISSN:1612-1872
Container-title:Chemistry & Biodiversity
language:en
Short-container-title:Chemistry & Biodiversity

Author:

Espino Ureña Marcos J.¹²³^ORCID,Katchborian‐Neto Albert⁴^ORCID,Trinidad Alexander Benítez²^ORCID,Ramírez Ramírez Maritza⁵^ORCID,Vásquez Tineo Manuel⁵^ORCID,Vilemar de Araújo‐Filho José⁶^ORCID,Ribeiro Wesley Lyeverton Correia⁷^ORCID,de Souza Collares Maia Castelo‐Branco Débora⁸^ORCID,de Oliveira Lorena Mayana Beserra⁶^ORCID,Bevilaqua Claudia Maria Leal⁶^ORCID,Chagas‐Paula Daniela Aparecida⁴^ORCID,Viegas Claudio⁹^ORCID,Marques Marcos José¹^ORCID

Affiliation:

1. Department of Pathology and Parasitology Institute of Biomedical Sciences Federal University of Alfenas Gabriel Monteiro da Silva St., 700 37130-001 Alfenas Minas Gerais Brazil

2. Animal Production Center Dominican Institute of Agricultural and Forestry Research Duarte Av. Km 24, Pedro Brand 340 parcel Santo Domingo. Dominican Republic

3. Faculty of Agronomic and Veterinary Sciences Autonomous University of Santo Domingo Camino de Engombe St. 10904 Santo Domingo Dominican Republic

4. Institute of Chemistry Federal University of Alfenas Gabriel Monteiro da Silva St., 700 37130-001 Alfenas Minas Gerais Brazil

5. Institute of Chemistry Science Faculty Autonomous University of Santo Domingo Alma Mater Av. Zona Universitaria Santo Domingo Dominican Republic

6. Laboratory of Parasitic Diseases Faculty of Veterinary State University of Ceará Dr. Silas Munguba Av., 1700, 60714–903 Fortaleza Ceará Brazil.

7. Department of Physiology and Pharmacology Federal University of Ceará Coronel Nunes deMelo St., 1127 60430-275 Rodolfo Teófilo, Fortaleza CE Brazil

8. Department of Pathology and Legal Medicine School of Medicine Federal University of Ceará Alexandre Baraúna St., 949 60430-160 Rodolfo Teófilo, Fortaleza CE Brazil

9. Laboratory of Research in Medicinal Chemistry – PeQuiM Institute of Chemistry Federal University of Alfenas Jovino Fernandes Sales Ave., 2600 37133-840 Alfenas Minas Gerais Brazil

Abstract

AbstractGastrointestinal nematode parasitism is a major burden to small ruminant production globally, compounded by increasing anthelmintic resistance. Previous studies have identified essential oils (EOs) from the Lippia genus with antiprotozoal and anthelmintic effects. Lippia dominguensis Moldenke (Ld), an endemic specie from the Dominican Republic, has similar popular uses, however, is chemically and pharmacologically yet uncharacterized. Here, we investigated the in vitro anthelmintic activity of LdEO and its ultrastructural effects on eggs and adult nematodes of Haemonchus contortus multidrug‐resistant isolated. The GC/MS analysis showed linalool (33.85 %), 1,8‐cineole (30.88 %), and δ‐terpineol (10.61 %) as the main EO constituents. The LdEO showed an IC50=0.523 mg/mL in the egg hatch test, and the motility in the adult worm motility test was 95.8 % at 1 mg/mL. The confocal scanning laser microscopy of eggs indicated permeabilization or disruption of egg cell membranes as the possible mechanism of action of LdEO. The scanning electron microscopy of adult worms showed wrinkling, undulations, and cuticular disruptions. The LdEO displayed significant in vitro anthelmintic activity on eggs and adult worms of H. contortus. Additionally, the LdEO showed low oral toxicity in mice at 2,000 mg/kg. Thus, additional in vivo studies are justified to determine its anthelmintic efficacy in small ruminants.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Wiley

Subject

Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbdv.202300135

Reference65 articles.

1. Water and small ruminant production

2. The critical importance of planned small ruminant livestock health and production in addressing global challenges surrounding food production and poverty alleviation

3. How to better account for livestock diversity and fodder seasonality in assessing the fodder intake of livestock grazing semi-arid sub-Saharan Africa rangelands

4. A. J. Aguilar-Caballero J. F. Torres-Acosta R. Cámara-Sarmiento C. Sandoval-Castro A. Ortega-Pacheco ‘La contribución del sector pecuario a la seguridad alimentaria en México’2013.