Affiliation:
1. Department of Veterinary Medicine College of Animal Sciences Zhejiang University 866 Yu Hang Tang Rd Hangzhou 310058 China
2. Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine MOA Key Laboratory of Animal Virology Center for Veterinary Sciences Zhejiang University Hangzhou 310058 China
Abstract
AbstractScrophulariae Radix (SR) has been widely used in Chinese herbal compound prescriptions, health care products and functional foods. The present study aimed to investigate the immunomodulatory activity of polysaccharides from SR (SRPs) in macrophages and explore the potential mechanisms. The results showed that four SRPs fractions (SRPs40, SRPs60, SRPs80 and SRPs100) had similar absorption peaks and monosaccharide compositions, but the intensities of absorption peaks and monosaccharide contents were distinguished. All SRPs fractions significantly enhanced the pinocytic activity, promoted the production of NO and TNF‐α, increased the mRNA expressions of inflammatory factors (IL‐1β, IL‐6, TNF‐α and PTGS2) and TLR2, and elevated the phosphorylation levels of p38, ERK, JNK, p65 and IκB. Moreover, the production of NO and TNF‐α stimulated by SRPs was dramatically suppressed by anti‐TLR2 antibody. These results indicated that SRPs activated macrophages through MAPK and NF‐κB signaling pathways via recognition of TLR2.
Funder
National Natural Science Foundation of China
Subject
Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering