Affiliation:
1. Department of Applied Chemistry Rajiv Gandhi Technological University Bhopal 462033 Madhya Pradesh India
2. Faculty of Pharmacy VNS Group of Institutions Bhopal 462044 Madhya Pradesh India
3. School of Pharmaceutical Sciences Rajiv Gandhi Technological University Bhopal India
4. University Grants Commission, New Delhi – 110002 New Delhi India
Abstract
AbstractDeciphering the most promising strategy for the evolution of potential wound‐healing therapeutics is one of the greatest challenging affairs to date. The development of peptide‐based smart scaffolds with innate antimicrobial, anti‐inflammatory, and antioxidant properties is an appealing way out. Aligned to the goal a set of Hydrogelators I–IV were developed utilizing the concept of chiral orchestration in diphenylalanine fragment, such that the most potent construct with all the bench marks namely mechanoresponsiveness, biocompatibility, consistent antimicrobial and antioxidant properties, could be fished out from the design. Interestingly, our in vitro Antifungal and Lipid peroxidation analysis identified the homochiral isomer Boc‐δ‐Ava‐L‐Phe‐L‐Phe‐OH (Hydrogelator I), as an ideal candidate for the wound healing experiment, so we proceeded for the in vivo histopathological and antioxidant measurements in Wister rats. Indeed the wound images obtained from the different sets of animals on the 14th day of treatment demonstrated that with increased recovery time, hydrogelator I displayed a significant reduction in the lesion diameter compared to the marketed drug, and negative control. Even the histopathological measurements using H & E staining demonstrated diminished tissue destruction, neutrophil infiltration necrosis, and lymphatic proliferation in the hydrogelators, in comparison to others, backed by in vivo lipid peroxidation data. Overall our investigation certifies hydrogelator I as an effective therapeutic for managing the wound healing complication.
Subject
Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering
Cited by
1 articles.
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