Affiliation:
1. Laboratory of Physiopathology Food and Biomolecules (LR-17-ES-03) Technology Center of Sidi Thabet University of Manouba 2020 Tunis Tunisia
2. Laboratory of Physiology Faculty of Medicine of Tunis University of Tunis El Manar La Rabta 1007 1006 Tunis Tunisia
3. Research Unit n° 17/ES/13 Faculty of Medicine University of Tunis El Manar 1067 Tunis Tunisia
4. Laboratory of Anatomy and Pathology AbderhamanMami Hospital 2080 Ariana Tunisia
5. Laboratory of Anatomy and Pathology Charles Nicole Hospital 1007 Tunis Tunisia
Abstract
AbstractIdiopathic pulmonary fibrosis (IPF) is a chronic interstitial pneumonia that leads to acute lung damage, deterioration of lung function, and increased mortality risk. In this study, we investigated the effects of the orange coproduct extract (OCE) and the combination of pure hesperidin and oleuropein (HO) on an experimental model of pulmonary fibrosis induced by bleomycin (BLM) in Wistar rats. Rats were divided into six groups: the control group (G1), the BLM group (G2), three groups (G3, G4, G5) receiving a single dose of BLM combined with OCE extract at 100, 200, and 300 mg/kg, and group 6 (G6) receiving a single dose of BLM combined with HO: both pure major phenolic compounds of OCE (hesperidin at 50 mg/kg) and olive leaves (oleuropein at 2.5 mg/kg). Oxidative stress in lung tissues was investigated using catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX) assays and the measurement of malondialdehyde (MDA) and lactate dehydrogenase (LDH) levels. Treatment with OCE and HO normalized the disturbance in oxidative markers′ levels and showed a significant reduction in fibrosis score with no renal or hepatic toxic effects. In conclusion, OCE and HO exhibit antifibrotic effects on a rat model of pulmonary fibrosis.