Affiliation:
1. Department of Molecular Biology and Genetics Faculty of Science Bartin University 74100 Bartin Turkey
2. Ulus Vocational School Department of Forestry and Forest Products Program Bartin University 74600 Bartin Turkey
3. Department of Chemistry Faculty of Science and Letters Tokat Gaziosmanpaşa University 60250 Tokat Turkey
Abstract
AbstractIn the present study, the main phytochemical components of endemic plant extracts and inhibitory potency were screened related to different biological activities. Seven compounds were quantified, and cyanidin‐3‐o‐glucoside was the dominant secondary metabolite in the extract of plants. The extract from P. asiae‐minoris (PAM) exhibited the best enzyme inhibitory activity against BChE (1.73±0.23 μg mL−1), tyrosinase (2.47±0.28 μg mL−1), α‐glucosidase (5.28±0.66 μg mL−1), AChE (8.66±0.86 μg mL−1), and ACE (19.27±1.02 μg mL−1). In vitro antioxidant assay, PAM extract possessed the highest activity in respect of DPPH radical scavenging (24.29±0.23 μg/mL), ABTS⋅+ scavenging (13.50±0.27 μg/mL) and FRAP reducing power (1.56±0.01 μmol TE/g extract). MIC values ranged from 1–8 mg/mL for antibacterial ability, and the PAM extract showed a stronger effect for B. subtilis, E. faecalis, and E. coli at 1 mg/mL. The antiproliferative ability of A. bartinense (AB) extract demonstrated a suppressive effect (IC50: 70.26 μg/mL) for pancreatic cancer cell lines. According to the affinity scores analysis, the cyanidin‐3‐o‐glucoside demonstrated the lowest docking scores against ACE, AChE, BChE, and collagenase. It was found that the PAM extract exhibited better inhibitory capabilities than A. bartinense. The P. asiae‐minoris plant, reported to be in the Critically Endangered (CR) category, should be conserved by culturing, considering its biological abilities.