Combination of Callyspongia sp. and Stem Cells for Wound Repair and Skin Regeneration: in Vivo and in Silico Evidences

Author:

Maher Zahran Eman1,Abdelwahab Miada F.2ORCID,Mohyeldin Reham H.3,Tammam Omar Y.4,Abdel‐Maqsoud Nehad M. Reda5,Altemani Faisal H.6,Algehainy Naseh A.6,Alanazi Mohammed A.6,Jalal Mohammed M.6,Elrehany Mahmoud A.7,Bringmann Gerhard8,Ramadan Abdelmohsen Usama12

Affiliation:

1. Department of Pharmacognosy Faculty of Pharmacy Deraya University Minia 61111 Egypt

2. Department of Pharmacognosy Faculty of Pharmacy Minia University Minia 61519 Egypt

3. Department of Pharmacology Faculty of Pharmacy Deraya University Minia 61111 Egypt

4. Department of Biochemistry Faculty of Pharmacy New Valley University New Valley Egypt

5. Department of Medical Science (Pathology) Deraya University Minia 61111 Egypt

6. Department of Medical Laboratory Technology Faculty of Applied Medical Sciences University of Tabuk Tabuk 71491 Saudi Arabia

7. Department of Biochemistry Faculty of Pharmacy Deraya University Minia 61111 Egypt

8. Institute of Organic Chemistry University of Würzburg Am Hubland 97074 Würzburg Germany

Abstract

AbstractDelayed healing of chronic wounds results in amputation and mortality rates in serious cases. The present study examines the merged wound‐restorative efficacy of injectable bone marrow‐derived mesenchymal stem cells (BMMSCs) and topical Callyspongia sp. extract in immunocompromised rats. HR‐LC–MS analysis of Callyspongia sp. extract tentatively identified twenty‐nine compounds (1–29) and highlighted its richness in fatty acids and terpenoids, known for their wound regenerating efficacies. The wound closure was greatly prominent in the BMMSCs/Callyspongia sp. group in contrast to the control group (p<0.001). The RT‐PCR gene expression emphasized these results by attenuating the oxidative, inflammatory, and immunity markers, further confirmed by histopathological findings. Additionally, in silico modeling was particularly targeting matrix metalloproteinase‐9 (MMP9), a key player in wound healing processes. Computational analysis revealed that compounds 18 and 19 potentially modulate MMP9 activity. The combination of BMMSCs and topical Callyspongia sp. extract holds a promise for regenerative therapy constituting a drastic advance in the wound cure of immunocompromised patients, eventually further safety assessments and clinical trials are required.

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3