Untargeted Metabolomic to Access Chemical Differences Induced by Dual Endophyte Cultures Isolated from <i>Euphorbia Umbellata</i>-Reference-Cited by-同舟云学术

Untargeted Metabolomic to Access Chemical Differences Induced by Dual Endophyte Cultures Isolated from Euphorbia Umbellata

Published:2024-05-10 Issue:6 Volume:21 Page:
ISSN:1612-1872
Container-title:Chemistry & Biodiversity
language:en
Short-container-title:Chemistry & Biodiversity

Author:

Santos Gusmão Amanda¹,Silva Conceição João Carlos¹^ORCID,Moreira de Queiros Santos Stefane¹,Lima Sampaio Pereira Cecilia¹,Siqueira de Almeida Chaves Douglas²,de Jesus Nicácio Karen³,Aparecida Chagas‐Paula Daniela⁴,de Oliveira Silva Eliane¹

Affiliation:

1. Departament of Organic Chemistry, Institute of Chemistry Federal University of Bahia Salvador 40170-115 Brazil

2. Pharmaceutical Science Department, Health and Biological Science Institute Federal Rural University of Rio de Janeiro Seropédica 23897-000 Brazil

3. Department of Chemistry Federal University of Mato Grosso Cuiabá 14040-901 Brazil

4. Chemistry Institute Federal University of Alfenas Alfenas 37130-001 Brazil

Abstract

AbstractEndophytic fungi live asymptomatically inside vegetal tissues, and such uncommon habitat contributes to their exceptional chemical diversity. Isolating natural products from endophytic fungi could fail due to silent biosynthetic gene clusters under ordinary in vitro culture conditions, and co‐culturing has been assayed to trigger their metabolism. We carried out single and dual cultures with 13 endophyte strains isolated from Euphorbia umbellata leaves. Multivariate statistics applied to untargeted metabolomics compared the chemical profiles of all endophyte cultures. PCA analysis guided the selection of the Aspergillus pseudonomiae J1 – Porogramme brasiliensis J9 dual culture for its most significant chemical differentiation: Five compounds were putatively annotated in the J1−J9 culture according to UHPLC‐HRMS data, kojic acid, haliclonol and its diastereoisomer, caffeic acid, and 2‐(3,4‐dihydroxyphenyl)acetaldehyde. Analysis by PLS‐DA using VIP score showed that kojic acid displayed the most significative importance in discriminating single and dual J1−J9 cultures.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbdv.202400395

Reference41 articles.

1. Investigation of Ehrlich ascites tumor cell death mechanisms induced by Synadenium umbellatum Pax.

2. Antitumoral and antiangiogenic activity of Synadenium umbellatum Pax

3. Endophytic Fungi as a Promising Source of Anticancer L-Asparaginase: A Review

4. Chemical ecology of fungi

5. Microbial Bioactive Compounds Produced by Endophytes (Bacteria and Fungi) and Their Uses in Plant Health