New Meroterpenoids and Anti‐Osteoclastogenic Polyketides from the Mangrove‐Derived fungus Arthrinium sp. SCSIO 41306

Abstract

AbstractTwo new meroterpenoids, arthrinones A and B (1 and 2), along with six known compounds (3–8), were obtained from the fungus Arthrinium sp. SCSIO 41306. Comprehensive methods such as chiral‐phase HPLC analysis and ECD calculations were applied to determine the absolute configurations. Griseofulvin (5), kojic acid (6), and 1H‐indole‐3‐carboxaldehyde (8) showed inhibition of NF‐κB in RAW 264.7 macrophages induced by lipopolysaccharide (LPS) with IC50 values of 22.21, 13.87 and 19.31 μM, respectively. In addition, griseofulvin (5) inhibited receptor activator of NF‐κB ligand (RANKL)‐induced osteoclast differentiation in a dose‐dependent manner without visible evidence of cytotoxicity in bone marrow macrophages (BMMs). This is the first report on the activity of griseofulvin (5) to inhibit osteoclast formation (IC50 10.09±0.21 μM).