Affiliation:
1. Laboratory of Pharmacology, Neurobiology, Anthropology and Environment Department of Biology Faculty of Sciences Semlalia University Cadi Ayyad BP 2390–40080 Marrakech Morocco
2. Laboratory of Agri-Food, Biotechnology, and Valorization of Plant Resources Phytochemistry and Pharmacology of Medicinal Plants Unit Faculty of Sciences Semlalia Cadi Ayyad University Marrakech Morocco
3. Higher Institute of Nursing Professions and Health Techniques Ministry of Health and Social Protection Beni Mellal Morocco
4. Laboratory of Physiopathology Genetic Molecular and Biotechnology Faculty of Sciences, Aïn Chock Hassan II University Casablanca Morocco
5. Laboratory of Genie Biologic Sultan Moulay Slimane University Faculty of Sciences and Technics Beni Mellal Moroocco
6. Department of Chemistry and Technologies of Drug Sapienza University P. le Aldo Moro 5 00185 Rome Italy
Abstract
AbstractAssessment of Moroccan Cannabis sativa Seed Oil: Chemical Analysis and Evaluation of Antioxidant, Toxicological, and Antinociceptive Effects. by K. Raoui et al., Cadi Ayyad University, Marrakech, Morocco. Cannabis sativa L., locally known as “El kif”, belongs to the Cannabaceae family. This study aims to conduct a chemical analysis of Cannabis sativa seed oil (CSSO) and assess its acute toxicity, antioxidant properties, and analgesic effects. The chemical analysis was performed using gas chromatography and mass spectrometry (GC/MS) to identify fatty acids (FAs) contents. Antioxidant activity was evaluated in vitro using the (2,2‐diphenyl‐1‐picrylhydrazyl) DPPH radical scavenging method and the (ferric reducing antioxidant power) FRAP method. Concurrently, acute toxicity, along with antinociceptive activity, was studied through three distinct animal models: writhing test, formalin test, and hot plate test. The results revealed that linoleic acid, oleic acid, α‐linolenic acid, and palmitic acid were the main components of CSSO. The LD50 of CSSO was greater than 5 g/kg, indicating low toxicity. Additionally, CSSO exhibited a significant content of flavonoids and total polyphenols, along with notable antioxidant activity with important values. The results indicated a significant increase in thermal stimulus latency, a reduction in the number of writhes induced by acetic acid, and a decrease in licking time in both phases of the formalin test. In conclusion, this study suggests promising results for CSSO, emphasizing its potential as a therapeutic agent.