Affiliation:
1. Institut des Sciences et Techniques Appliquées Université Saâd Dahlab Blida n° 6 Chemin Javel 09000 Blida Algérie
2. Laboratoire des Produits Naturels Département de Biologie Université Abou Bekr Belkaïd Mansourah Imama 13000 Tlemcen Algérie
3. Laboratoire d'Ecologie et Gestion des Ecosystèmes Naturels Département d'Ecologie et Environnement Université Abou Bekr Belkaïd, Imama 13000 Tlemcen Algérie.
4. Université de Corse-CNRS, UMR 6134 SPE Route des Sanguinaires 20000 Ajaccio France
Abstract
AbstractThe chemical composition of 33 oil samples isolated from the berries of individual trees of two subspecies of Juniperus oxycedrus L., collected in four locations in Tlemcen and Aïn Temouchent provinces, was investigated by GC‐FID, GC/MS and 13CNMR. In total, 83 compounds were identified, accounting for 85.9 % to 98.6 % of the total composition. Yields ranged between 0.02 and 0.40 % (w/w). All compositions were submitted to statistical analysis. Combination of hierarchical clustering dendrogram and principal component analysis suggested the existence of two groups, the second group being sub‐divided into two sub‐groups. Groups and sub‐groups were distinguished with respect to their contents of α‐pinene, germacrene D, myrcene and, to a lesser extent, of manoyl oxide. The chemical composition of the oils belonging to subsp. oxycedrus was largely dominated by monoterpenes. α‐Pinene (23.7–63.2 %) and germacrene D (4.5–25.4 %) were the major components, followed by myrcene (3.1–16.7 %). Conversely, samples oils of subsp. macrocarpa were largely dominated by germacrene D (4.4–30.0 %), followed by a lower content of α‐pinene (10.0–24.3 %) and myrcene (1.0–4.3 %) and a significant content of manoyl oxide (4.3–9.9 %).