Study on Calceolarioside A Anti‐Aβ25–35‐Induced Damage in SH‐SY5Y cells by Atomic Force Microscopy

Author:

Chen Yujuan123ORCID,Qu Kaige23,Wang Ying23,Zhang Qinghan13,Xie Ying13,Wang Zuobing234

Affiliation:

1. School of Life Sciences Changchun University of Science and Technology Changchun 130022 China

2. International Research Center for Nano Handling and Manufacturing of China Changchun University of Science and Technology Changchun 130022 China

3. Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing Changchun University of Science and Technology Changchun 130022 China

4. JR3CN & IRAC University of Bedfordshire Luton LU1 3JU UK

Abstract

AbstractCalceolarioside A is a phenylethyl glycoside compound, originally isolated from the bark of Fraxinus mandshurica Rupr. In this work, the protective effect of Calceolarioside A on beta Amyloid protein induced toxicity in SH‐SY5Y cells was studied. DPPH experiment, MTT assay, SEM, Atomic force microscopy and Colony formation were used to study the activity of Calceolarioside A. The results in this article show that the survival rate of the cells with Calceolarioside A (20–40 mg/mL) was significantly higher than that of the model cells without Calceolarioside A. Calceolarioside A could protect SH‐SY5Y cells by improving some parameters in cells, such as the cell height, Young's modulus, adhesion and branch. In summary, Calceolarioside A can reduce Aβ25–35‐induced damage in SH‐SY5Y cells. It can be a potential medicine to treatment with AD.

Publisher

Wiley

Subject

Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering

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