Affiliation:
1. Department of Pharmacognosy Faculty of Pharmacy Ataturk University Erzurum Turkey
2. Medicinal and Aromatic Plant and Drug Research Center Ataturk University Erzurum Turkey
3. Department of Biology Faculty of Science Ataturk University Erzurum Turkey
4. Department of Pharmaceutical Botany Faculty of Pharmacy Ataturk University Erzurum Turkey
5. Department of Pharmacy and Pharmaceutical Services Igdir University Igdir Turkey
Abstract
AbstractThis study examined the effects of methanol extract and its sub‐extracts from Epilobium angustifolium on α‐glucosidase and α‐amylase activity. Secondary metabolites and amino acids were quantified using LC–MS/MS. Dichloromethane sub‐extract displayed the highest activity and was chosen for further investigation. Despite the widespread use of E. angustifolium, genotoxicity studies were conducted to assess its safety. Dichloromethane significantly inhibited α‐glucosidase (IC50=17.340 μg/mL), making it approximately 293 times more effective than acarbose. Six known compounds, including gallic acid (1), a mixture of quercetin‐3‐O‐α‐galactoside (2a) and quercetin‐3‐O‐α‐glucoside (2b), quercetin‐3‐O‐α‐glucuronic acid (3), quercetin‐3‐O‐α‐rhamnoside (4), and kaempferol‐3‐O‐α‐rhamnoside (5) were identified. Quercetin‐3‐O‐α‐rhamnoside exhibited the highest inhibition of α‐glucosidase (IC50=1735±85 μM), making it 3.70 times more effective than acarbose. Dichloromethane also showed significant antigenotoxic activity against mutagenesis induced by NaN3, 9‐AA, 4‐NPD, and MNNG. Gallic acid was found in the highest abundance (13253.6931 ng/mL) in the methanolic extract. Furthermore, L‐Aspartic acid was the most concentrated amino acid (363.5620 nmol/mL) in the methanolic extract.
Subject
Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering
Cited by
1 articles.
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