Affiliation:
1. Laboratory of Pharmacology and Molecular Chemistry (LFQM) Department of Biological Chemistry Regional University of Cariri (URCA) 63105-000 Crato CE Brazil
2. Oswaldo Cruz Foundation (Fiocruz) Fiocruz Ceará 60180-900 Eusébio CE Brazil
3. Natural Products Pharmacology Laboratory Department of Biological Chemistry Regional University of Cariri (URCA) 63105-000 Crato CE Brazil
4. Molecular Biology Departament Federal University of Paraiba (UFPB) Campus I – Jardim Cidade Universitária 58059-900 João Pessoa PB Brazil
Abstract
AbstractPsidium brownianum Mart is reported in the literature by antinociceptive and antioxidant activities, indicating that this species′ secondary metabolites might be used to control inflammatory processes. The present study aimed to characterize the topical antiedematogenic activity of the essential oil of Psidium brownianum Mart. (OEPB) in ear edema models by different inflammatory agents. Female Swiss mice (25–35 g) and Wistar albino rats (200–300 g) were used throughout tests (n=6/group) on acute or chronic edema models induced by single and multiple topical applications. The OEPB is administered topically pure or at a concentration of 100 or 200 mg/mL. The antiedematogenic mechanism of OEPB was analyzed by administering capsaicin, arachidonic acid, histamine, and phenol at the best effective dose (200 mg/mL). The results showed a significant reduction of edema‐induced single (28.87 %) and multiple (50.13 %) applications of croton oil compared to the negative control group. Regarding potential mechanisms of action, OEPB (200 mg/mL) inhibited the development of edema triggered by capsaicin (29.95 %), arachidonic acid (22.66 %), phenol (23.35 %), and histamine (75.46 %), suggesting an interference with the histaminergic pathway. These results indicate that OEPB presents a topical antiedematogenic effect in acute and chronic murine models, possibly interfering with inflammatory pathways triggered by mediators such as histamine.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Financiadora de Estudos e Projetos