Investigating the Anticancer Effects of Pleurotus ostreatus Polysaccharide on G0/G1 Cell Cycle Arrest and Apoptosis in Ehrlich Ascites Carcinoma Cells

Abstract

AbstractCancer is one of the leading causes of mortality worldwide. Despite the advancement of cancer treatment by various means including surgery, chemotherapy etc, cancer is still a challenging disease to manage. This study was undertaken to investigate extraction, purification, structural elucidation, and the potential anti‐cancer effects of Pleurotus ostreatus polysaccharide (POP). The anti‐cancer activities were performed on the Ehrlich Ascites Carcinoma Cell Line. The results demonstrated that the MW of POP was154649.8 Da with homopolysaccharide composed of D‐glucose units, featuring (1→6)‐α‐D‐Glcp backbone with O‐6 branches and T‐α‐D‐Glcp terminations. and the yield was 6.27 %. The antitumor activity assessment demonstrated significant cytotoxicity of POP against Ehrlich Ascites Carcinoma (EAC) cells, with an IC50 of 121.801 μg mL, supported by LDH release analysis. POP inhibited cell migration, invasion, and colony formation, indicating its potential as an anti‐cancer agent. POP elicited the apoptotic activity with the upregulation of Caspase‐9 and Bax, and downregulation of Bcl‐2. The DNA fragmentation assay further confirmed apoptosis‐mediated DNA degradations. Additionally, POP‐induced cell cycle arrest at the G0/G1 phase, by altering the expression of p53, Cyclin D, and Cdk4 proteins. So, Pleurotus ostreatus polysaccharide (POP) showed significant cytotoxicity on Ehrlich Ascites Carcinoma cells, indicating potential as an anti‐cancer agent.