Overview of Digital Electrophoresis Analysis

Author:

Wang Qi1,Chang Neil1,Moomaw Butch2,Medberry Scott3,Gallagher Sean R.1

Affiliation:

1. Analytik Jena US LLC Upland California

2. Hamamatsu Photonic Systems Spring Branch Texas

3. Model N, Inc. Redwood Shores California

Abstract

AbstractDigital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using sCMOS and CCD‐based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of technologies used for image capture, and provides an introduction to the software and methods used for one‐ and two‐dimensional digital image analysis. © 2023 Wiley Periodicals LLC.

Publisher

Wiley

Subject

Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference26 articles.

1. The MELANIE project: From a biopsy to automatic protein map interpretation by computer

2. A method for the construction of minimum‐redundancy codes;Huffman D. A.;Proceedings of the Institute of Radio Engineers,1952

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