Gene optimization mechanisms: A multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli

Author:

Maertens Barbara,Spriestersbach Anne,von Groll Uritza,Roth Udo,Kubicek Jan,Gerrits Michael,Graf Marcus,Liss Michael,Daubert Daniela,Wagner Ralf,Schäfer Frank

Funder

Bundesministerium für Bildung und Forschung

Publisher

Wiley

Subject

Molecular Biology,Biochemistry

Reference35 articles.

1. Protein production and purification;Gräslund;Nat Methods,2008

2. Advances in Escherichia coli production of therapeutic proteins;Swartz;Curr Opin Biotechnol,2001

3. Codon usage tabulated from the international DNA sequence databases: status from the year 2000;Nakamura;Nucl Acids Res,2000

4. Enhanced expression of a recombinant malaria candidate vaccine in Escherichia coli by codon optimization;Zhou;Protein Expr. Purif.,2004

5. Influence of messenger RNA secondary structure on translation efficiency;Nomura;Nucleic Acids Symp. Ser.,1984

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