Monitoring cell productivity for the production of recombinant proteins by flow cytometry: An effective application using the cold capture assay
Author:
Affiliation:
1. Institute of Technical Chemistry Leibniz University Hannover Hannover Germany
2. Sartorius Stedim Biotech GmbH Göttingen Germany
Funder
Deutsche Forschungsgemeinschaft
Publisher
Wiley
Subject
Bioengineering,Environmental Engineering,Biotechnology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/elsc.202000049
Reference18 articles.
1. A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity
2. Selection of CHO host cell subclones with increased specific antibody production rates by repeated cycles of transient transfection and cell sorting
3. Flow-cytometry and cell sorting: An efficient approach to investigate productivity and cell physiology in mammalian cell factories
4. A study on the temperature dependency and time course of the cold capture antibody secretion assay
5. The art of CHO cell engineering: A comprehensive retrospect and future perspectives
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