Affiliation:
1. Department of Biomedical Engineering Institute for Complex Molecular Systems (ICMS) Eindhoven University of Technology PO Box 513 Eindhoven 5600 MB The Netherlands
2. School of Pharmacy University of Nottingham University Park Nottingham NG7 2RD UK
3. Department of Medical Microbiology and Infection Prevention Amsterdam institute for Infection and Immunity Amsterdam University Medical Centers University of Amsterdam Amsterdam 1105 AZ The Netherlands
Abstract
AbstractA polymer microarray based on the supramolecular ureido‐pyrimidinone (UPy) moiety is fabricated to screen antimicrobial materials for their ability to support cell adhesion. UPy‐functionalized additives, either cell‐adhesive, antimicrobial or control peptides, are used, and investigated in different combinations at different concentrations, resulting in a library of 194 spots. These are characterized on composition and morphology to evaluate the microarray fabrication. Normal human dermal fibroblasts are cultured on the microarrays and cell adhesion to the spots is systematically analyzed. Results demonstrate enhanced cell adhesion on spots with combinations including the antimicrobial peptides. This study clearly proves the power of the high throughput approach in combination with supramolecular molecules, to screen additive libraries for desired biological response.
Funder
Dutch Polymer Institute
HORIZON EUROPE Widening Participation and Strengthening the European Research Area
Ministerie van Onderwijs, Cultuur en Wetenschap