Affiliation:
1. Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science & Technology Normal University Nanchang China
2. Departments of Pharmaceutical Analysis, School of Pharmacy Qingdao University Medical College Qingdao China
3. Institute of Carbon Neutral New Energy Research Yuzhang Normal University Nanchang China
Abstract
Hypochlorous acid (HClO) and its ionic form (ClO−), as important components of reactive oxygen species, were produced mainly in mitochondria and participated in various biological processes, and they abnormal level could cause various diseases and threaten human health. Hence, a “turn‐on” phosphorescent probe Ir‐DAMN based on Ir (III) complex was synthesized to monitor HClO/ClO− in DMF/PBS solution and living cells. Due to isomerization of C=N bond, probe Ir‐DAMN exhibited very weak phosphorescence initially, which displayed obvious phosphorescent enhancement at 620 nm after upon addition with ClO−. Confocal bioimaging experiments implied that probe Ir‐DAMN possessed good cell membrane permeability, specific localization of mitochondria and could achieve detection of exogenous and endogenous HClO/ClO− in the living cells.
Funder
National Natural Science Foundation of China