Harmonization and standardization of nucleus pulposus cell extraction and culture methods-Reference-Cited by-同舟云学术

Harmonization and standardization of nucleus pulposus cell extraction and culture methods

Published:2023-01-10 Issue:1 Volume:6 Page:
ISSN:2572-1143
Container-title:JOR SPINE
language:en
Short-container-title:JOR Spine

Author:

Basatvat Shaghayegh¹^ORCID,Bach Frances C.²^ORCID,Barcellona Marcos N.³^ORCID,Binch Abbie L.¹,Buckley Conor T.³^ORCID,Bueno Brian⁴^ORCID,Chahine Nadeen O.⁵^ORCID,Chee Ana⁶^ORCID,Creemers Laura B.⁷^ORCID,Dudli Stefan⁸^ORCID,Fearing Bailey⁹^ORCID,Ferguson Stephen J.¹⁰^ORCID,Gansau Jennifer⁴^ORCID,Gantenbein Benjamin¹¹¹²^ORCID,Gawri Rahul¹³¹⁴^ORCID,Glaeser Juliane D.¹⁵,Grad Sibylle¹⁶^ORCID,Guerrero Julien¹¹¹⁷^ORCID,Haglund Lisbet¹³^ORCID,Hernandez Paula A.¹⁸^ORCID,Hoyland Judith A.¹⁹^ORCID,Huang Charles²⁰^ORCID,Iatridis James C.⁴^ORCID,Illien‐Junger Svenja²¹^ORCID,Jing Liufang²¹²²,Kraus Petra²¹²³^ORCID,Laagland Lisanne T.²^ORCID,Lang Gernot²⁴,Leung Victor²⁵^ORCID,Li Zhen¹⁶^ORCID,Lufkin Thomas²³^ORCID,van Maanen Josette C.²,McDonnell Emily E.³^ORCID,Panebianco Chris J.⁴,Presciutti Steven M.²¹^ORCID,Rao Sanjna⁴^ORCID,Richardson Stephen M.¹⁹^ORCID,Romereim Sarah⁹,Schmitz Tara C.²⁶^ORCID,Schol Jordy²⁷^ORCID,Setton Lori²⁸^ORCID,Sheyn Dmitriy¹⁵^ORCID,Snuggs Joseph W.¹^ORCID,Sun Y.²⁵^ORCID,Tan Xiaohong²²,Tryfonidou Marianna A.²^ORCID,Vo Nam²⁹^ORCID,Wang Dong²⁹^ORCID,Williams Brandon⁶,Williams Rebecca¹^ORCID,Yoon S. Tim²¹^ORCID,Le Maitre Christine L.¹³⁰^ORCID

Affiliation:

1. Biomolecular Sciences Research Centre Sheffield Hallam University Sheffield UK

2. Department of Clinical Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands

3. Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin The University of Dublin Dublin Ireland

4. Leni & Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA

5. Departments of Orthopedic Surgery and Biomedical Engineering Columbia University New York New York USA

6. Department of Orthopedic Surgery Rush University Medical Center Chicago Illinois USA

7. Department of Orthopedics University Medical Center Utrecht Utrecht The Netherlands

8. Center for Experimental Rheumatology University of Zurich Zurich Switzerland

9. Department of Orthopedic Surgery Atrium Health Musculoskeletal Institute Charlotte North Carolina USA

10. Institute for Biomechanics ETH Zurich Zurich Switzerland

11. Bone & Joint Program, Department for BioMedical Research (DBMR), Medical Faculty University of Bern Bern Switzerland

12. Department for Orthopedics and Traumatology, Insel University Hospital University of Bern Bern Switzerland

13. Division of Orthopaedic Surgery, Department of Surgery McGill University Montreal Canada

14. Regenerative Orthopaedics and Innovation Laboratory McGill University Montreal Canada

15. Cedars‐Sinai Medical Center Los Angeles California USA

16. AO Research Institute Davos Davos Switzerland

17. Center of Dental Medicine, Oral Biotechnology & Bioengineering University of Zurich Zurich Switzerland

18. Department of Orthopaedic Surgery University of Texas Southwestern Medical Center Dallas Texas USA

19. School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Sciences Centre The University of Manchester Manchester UK

20. Department of Biomedical Engineering University of Miami Coral Gables Florida USA

21. Department of Orthopaedics Emory University School of Medicine Atlanta GA USA

22. Department of Biomedical Engineering Washington University in St. Louis St. Louis Missouri USA

23. Department of Biology Clarkson University Potsdam New York USA

24. Department of Orthopedics and Trauma Surgery, Medical Center, Faculty of Medicine Albert‐Ludwigs‐University of Freiburg Freiburg im Breisgau Germany

25. Department of Orthopaedics & Traumatology The University of Hong Kong Hong Kong SAR China

26. Orthopaedic Biomechanics, Department of Biomedical Engineering Eindhoven University of Technology Eindhoven The Netherlands

27. Department of Orthopedic Surgery Tokai University School of Medicine Isehara Japan

28. Departments of Biomedical Engineering and Orthopedic Surgery Washington University in St. Louis St. Louis Missouri USA

29. Department of Orthopaedic Surgery University of Pittsburgh Pittsburgh Pennsylvania USA

30. Department of Oncology and Metabolism University of Sheffield Sheffield South Yorkshire UK

Abstract

AbstractBackgroundIn vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab‐to‐lab variability jeopardizes the much‐needed progress in the field. Here, an international group of spine scientists collaborated to standardize extraction and expansion techniques for NP cells to reduce variability, improve comparability between labs and improve utilization of funding and resources.MethodsThe most commonly applied methods for NP cell extraction, expansion, and re‐differentiation were identified using a questionnaire to research groups worldwide. NP cell extraction methods from rat, rabbit, pig, dog, cow, and human NP tissue were experimentally assessed. Expansion and re‐differentiation media and techniques were also investigated.ResultsRecommended protocols are provided for extraction, expansion, and re‐differentiation of NP cells from common species utilized for NP cell culture.ConclusionsThis international, multilab and multispecies study identified cell extraction methods for greater cell yield and fewer gene expression changes by applying species‐specific pronase usage, 60–100 U/ml collagenase for shorter durations. Recommendations for NP cell expansion, passage number, and many factors driving successful cell culture in different species are also addressed to support harmonization, rigor, and cross‐lab comparisons on NP cells worldwide.

Funder

Dutch Arthritis Association

H2020 European Institute of Innovation and Technology

Irish Research Council for Science, Engineering and Technology

National Science Foundation

AOSpine

National Institute of Arthritis and Musculoskeletal and Skin Diseases

Publisher

Wiley

Subject

Orthopedics and Sports Medicine

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