Surface plasmon resonance aptasensing and computational analysis of Staphylococcus aureusIsdA surface protein

Author:

Bruce‐Tagoe Tracy Ann1,Harnish Michael T.2,Soleimani Shokoufeh3,Ullah Najeeb1,Shen Tongye2,Danquah Michael K.1ORCID

Affiliation:

1. Department of Chemical and Biomolecular Engineering University of Tennessee Knoxville Tennessee USA

2. Department of Biochemistry & Cellular and Molecular Biology University of Tennessee Knoxville Tennessee USA

3. Department of Mechanical, Aerospace and Biomedical Engineering University of Tennessee Knoxville Tennessee USA

Abstract

AbstractStaphylococcus aureus (S. aureus), a common foodborne pathogen, poses significant public health challenges due to its association with various infectious diseases. A key player in its pathogenicity, which is the IsdA protein, is an essential virulence factor in S. aureus infections. In this work, we present an integrated in‐silico and experimental approach using MD simulations and surface plasmon resonance (SPR)‐based aptasensing measurements to investigate S. aureus biorecognition via IsdA surface protein binding. SPR, a powerful real‐time and label‐free technique, was utilized to characterize interaction dynamics between the aptamer and IsdA protein, and MD simulations was used to characterize the stable and dynamic binding regions. By characterizing and optimizing pivotal parameters such as aptamer concentration and buffer conditions, we determined the aptamer's binding performance. Under optimal conditions of pH 7.4 and 150 mM NaCl concentration, the kinetic parameters were determined; ka = 3.789 × 104/Ms, kd = 1.798 × 103/s, and KD = 4.745 × 10−8 M. The simulations revealed regions of interest in the IsdA‐aptamer complex. Region I, which includes interactions between amino acid residues H106 and R107 and nucleotide residues 9G, 10U, 11G and 12U of the aptamer, had the strongest interaction, based on ΔG and B‐factor values, and hence contributed the most to the stability of the interaction. Region II, which covers residue 37A reflects the dynamic nature of the interaction due to frequent contacts. The approach presents a rigorous characterization of aptamer‐IsdA binding behavior, supporting the potential application of the IsdA‐binding aptamer system for S. aureus biosensing.

Funder

National Science Foundation

Publisher

Wiley

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