Affiliation:
1. National Resource Centre for Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing P. R. China
2. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao‐di Herbs, National Resource Centre for Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing P. R. China
3. Key Laboratory of Biology and Cultivation of Herb Medicine Ministry of Agriculture and Rural Affairs Beijing P. R. China
4. Pharmacy Faculty Hubei University of Chinese Medicine Wuhan P. R. China
Abstract
AbstractIntroductionAgarwood, a fragrant resinous wood mainly formed by Aquilaria spp., is used worldwide as a natural fragrance and traditional medicine. A large amount of Aquilaria sinensis (Lour.) Gilg leaves are underutilised during the process of the agarwood industry, and the development of A. sinensis leaves as tea has recently attracted more and more attention. However, the small molecule profile of A. sinensis leaves and their bioactivities has been rarely reported.ObjectiveTo conduct a rapid untargeted liquid chromatography–mass spectrometry (LC–MS) analysis of A. sinensis leaves with a molecular networking (MN) strategy and evaluate its antioxidant and antidiabetic value.MethodA MN‐assisted tandem mass spectrometry (MS/MS) analysis strategy was used to investigate the small molecule profile of A. sinensis leaves. Additionally, the integration of antioxidant and α‐glucosidase inhibitory assays with MN analysis was executed to expeditiously characterise the bioactive compounds for potential prospective application.ResultsFive main chemical groups including phenol C‐glycosides, organic acids, 2‐(2‐phenylethyl) chromones, benzophenone O‐glycosides and flavonoids were rapidly revealed from the A. sinensis leaves. Eighty‐one compounds were provisionally identified by comparing their MS/MS fragments with canonical pathways. The featured xanthone C‐glycosides and benzophenone C‐glycosides were recognised as the primary components of A. sinensis leaves. Several dimers and a trimer of mangiferin were reported firstly in A. sinensis leaves. Furthermore, 17 and 14 potential bioactive molecules were rapidly annotated from antioxidant and α‐glucosidase inhibitory fraction, respectively.ConclusionOur findings will help expand the utilisation of A. sinensis leaves and thus promote the high‐quality development of agarwood industry.
Subject
Complementary and alternative medicine,Drug Discovery,Plant Science,Molecular Medicine,General Medicine,Biochemistry,Food Science,Analytical Chemistry
Reference30 articles.
1. Research progress and quality influencing factors of Aquilaria sinensis leaves;Wang X;Chinese J Trop Agric,2021
2. Material selection and quality analysis of Aquilaria sinensis leaf tea;Wang Z‐H;Food Res Dev,2021
3. Feeding Aquilaria sinensis Leaves Modulates Lipid Metabolism and Improves the Meat Quality of Goats