Identification and quantification of the antioxidants in Ginkgo biloba leaf

Author:

Hu Chenxiu12,Wang Yujing1,Deng Yingqian1,Yao Jianbiao3,Min Hui3,Hu Jiqiang3,Fan Xiaohui12,Wang Shufang12ORCID

Affiliation:

1. College of Pharmaceutical Sciences Zhejiang University Hangzhou China

2. Innovation Center of Translational Pharmacy Jinhua Institute of Zhejiang University Jinhua China

3. Zhejiang Conba Pharmaceutical Co., Ltd Hangzhou China

Abstract

AbstractThe antioxidant activity of Ginkgo biloba leaf (GBL) extract is closely related to its efficacy against various diseases; however, the antioxidant activities of the specific constituents of GBL remain unclear. In this study, 194 GBL constituents were identified using ultra‐performance liquid chromatography‐quadrupole‐time‐of‐flight mass spectrometry, including 97 flavonoids, 37 terpenoids, 29 lignans, 19 carboxylic acids, 5 alkylphenolic acids, 5 alkylphenols, and 2 other compounds. The cleavage rules of the main constituents of GBL were dissected in detail. The 36 GBL constituents with high antioxidant activity were subsequently discovered using the oxygen radical absorbance capacity assay, including 30 flavonoids and six carboxylic acids. Finally, an HPLC analysis method was established to determine the content of the nine major antioxidants in the three batches of GBL. Among them, kaempferol 3‐O‐β‐D‐(6″‐p‐coumaroyl) glucopyranosyl‐(1‐2)‐α‐L‐rhamnopyranoside, kaempferol‐3‐O‐rutinoside, and rutin exhibited high antioxidant activity and were found in significant amounts in GBL, with concentrations greater than 0.7 mg/g. These results provide an important reference for the development of pharmaceuticals and health products containing GBL.

Funder

Science and Technology Program of Zhejiang Province

Natural Science Foundation of Zhejiang Province

Publisher

Wiley

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