Affiliation:
1. Department of Pharmacy, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
2. Department of Natural Medicinal Chemistry School of Pharmacy Naval Medical University Shanghai China
3. Department of Pharmaceutical Analysis, School of Pharmacy Shandong University of Traditional Chinese Medicine Jinan China
Abstract
AbstractCistanche tubulosa (CT), a well‐known traditional Chinese medicine, has always been processed with rice wine for the treatment of kidney‐yang deficiency syndrome (KYDS) since time immemorial. To explore the effect of processing on the efficacy and metabolites of CT in vivo, a comprehensive method using ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry was established for the analysis of the altered endogenous metabolites in response to the intervention of the raw and processed CT in KYDS model and the metabolites of the absorbed compounds in rats after gastric perfusion. It was shown that CT could improve KYDS, and the effect of the processed product was more significant. A total of 47 differential metabolites were identified in urine. Pathway analysis proved that purine metabolism; alanine, aspartate, and glutamate metabolism; and citrate cycle were the main pathways. Furthermore, 53 prototypes and 48 metabolites have been detected in rats. This was the first systematic research focus on the metabolites of raw and processed CT in vivo, which could provide a scientific basis for explaining the increasing efficiency of the processed CT. Moreover, it provides a valuable strategy for analyzing the chemical components and metabolites of other TCM prescriptions.
Funder
National Key Research and Development Program of China Stem Cell and Translational Research
National Major Science and Technology Projects of China
National Natural Science Foundation of China
Research and Development
Science and Technology Commission of Shanghai Municipality
Subject
Clinical Biochemistry,Drug Discovery,Pharmacology,Molecular Biology,General Medicine,Biochemistry,Analytical Chemistry