Affiliation:
1. State Key Laboratory for Manufacturing Systems Engineering Xi'an Jiaotong University Xi'an 710049 P. R. China
2. NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices Xi'an Jiaotong University Xi'an 710049 P. R. China
Abstract
AbstractBioprinting has attracted extensive attention in the field of tissue engineering due to its unique capability in constructing biomimetic tissue constructs in a highly controlled manner. However, it is still challenging to reproduce the physical and structural properties of native electroactive tissues due to the poor electroconductivity of current bioink systems as well as the limited printing resolution of conventional bioprinting techniques. In this work, an electro‐conductive hydrogel is prepared by introducing poly (3,4‐ethylene dioxythiophene): poly (styrene sulfonate) (PEDOT: PSS) into an RGD (GGGGRGDSP)‐functionalized alginate and fibrin system (RAF), and then electrohydrodynamic (EHD)‐bioprinted to form living tissue constructs with microscale resolution. The addition of 0.1 (w/v%) PEDOT: PSS increases the electroconductivity to 1.95 ± 0.21 S m−1 and simultaneously has little effect on cell viability. Compared with pure RAF bioink, the presence of PEDOT: PSS expands the printable parameters for EHD‐bioprinting, and hydrogel filaments with the smallest feature size of 48.91 ± 3.44 µm can be obtained by further optimizing process parameters. Furthermore, the EHD‐bioprinted electro‐conductive living tissue constructs with improved resolution show good viability (>85%). The synergy of the advanced electro‐conductive hydrogel and EHD‐bioprinting presented here may provide a promising approach for engineering electro‐conductive and cell‐laden constructs for electroactive tissue engineering.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Natural Science Basic Research Program of Shaanxi Province
China Postdoctoral Science Foundation
Cited by
4 articles.
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