Affiliation:
1. Department of Stomatology, Huashan Hospital Fudan University Shanghai 200001 P. R. China
2. Shanghai Key Laboratory of Craniomaxillofacial Development and Disease, Shanghai Stomatological Hospital Fudan University Shanghai 200001 P. R. China
Abstract
AbstractCPNE1 is a calcium‐dependent, phospholipid‐binding protein that is ubiquitously expressed in various tissues and organs. This study investigates the expression and localization of CPNE1 in tooth germ development and the role of CPNE1 in odontoblastic differentiation. In rat tooth germs, CPNE1 is expressed in the odontoblasts and ameloblasts since the late bell stage. The depletion of CPNE1 in the stem cells from apical papilla (SCAPs) clearly inhibits the expression of odontoblastic‐related genes and the formation of mineralized nodules during differentiation, while CPNE1 overexpression promotes this process. In addition, CPNE1 overexpression increases AKT phosphorylation during the odontoblastic differentiation of SCAPs. Furthermore, treatment with AKT inhibitor (MK2206) reduces the expression of odontoblastic‐related genes in CPNE1 over‐expressed SCAPs, and Alizarin Red staining shows reduced mineralization. These results suggest that CPNE1 plays a role in the tooth germ development as well as the odontblastic differentiation of SCAPs in vitro that is related to the AKT signaling pathway.
Funder
National Natural Science Foundation of China
Subject
General Biochemistry, Genetics and Molecular Biology,Biomedical Engineering,Biomaterials