Affiliation:
1. Department of Pharmaceutics, School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, UK
Abstract
Abstract
Egg phosphatidylcholine liposomes were freeze-dried in the presence and absence of trehalose. The lyophilized liposomes were rehydrated and aerosolized using a Pari LC jet nebulizer. The size of the aerosols generated was determined by laser diffraction, which was also used to determine the size distribution of the liposomes before lyophilization, post-rehydration, in the nebulizer post-aerosolization and those deposited in the two stages of a twin impinger. In the absence of trehalose, large liposomes and vesicle aggregates were produced on rehydration, which were rapidly reduced in size on nebulization. Liposomes with a mean size of 1 or 2.5 μm, freeze-dried with trehalose, had a mean size less than 3 μm following rehydration and exhibited enhanced stability to nebulization. Liposomes of 1 μm before freeze-drying were evenly distributed within aerosols generated by the nebulizer, whilst aerosols generated from 2.5 μm liposomes were fractionated in the twin impinger with the largest liposomes collected in the upper stage.
Publisher
Oxford University Press (OUP)
Subject
Pharmaceutical Science,Pharmacology
Reference17 articles.
1. Preparation and use of liposomes as models of biological membranes;Bangham;Methods Membr. Biol.,1974
2. Nebulisers for the generation of liposomal aerosols;Bridges;Int. J. Pharm.,1998
3. Factors influencing the jet nebulization of liposomes;Bridges;Int. J. Pharm.,2000
4. Stability of liposomes on storage: freeze-dried, frozen or as an aqueous dispersion;Crommelin;Pharm. Res.,1984
5. Preservation of liposomes by freeze-drying;Crowe,1992
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