Impairment of the renal p-aminohippurate transport induced by 6-hydroxydopamine

Abstract

Abstract In this study, the effects of 6-hydroxydopamine (6-OHDA) on renal p-aminohippurate transport were investigated in-vitro using rat renal cortical slices. Cisplatin, a known nephrotoxin, was used as positive control. Renal cortical slices were incubated for 60 min in a cisplatin-containing medium (0.83–5.0 μm) at 37°C under a 100% O2 atmosphere. In another series of experiments, renal cortical slices were incubated in a 3.33 μm cisplatin-containing medium for 15–120min or in a cisplatin-free medium. Subsequently, for each series of experiments, kidney slices were incubated at 25°C for 90 min in a media containing p-aminohippurate. In a further series of experiments, renal cortical slices were incubated for 60 min in a 6-OHDA containing medium (3.125–100 μm) at 37°C under a 100% O2 atmosphere. In another series of experiments, renal cortical slices were incubated in a 50 μm 6-OHDA-containing medium for 15–120min or in 6-OHDA-free medium. Subsequently, for each series of experiments, kidney slices were incubated at 25°C for 90 min in a media containing p-aminohippurate. The results of this study where slices were incubated in 6-OHDA- or cisplatin-containing media indicate that both 6-OHDA and cisplatin induced a time- and concentration-dependent decrease in p-aminohippurate accumulation by renal cortical slices. Therefore, similarly to cisplatin, 6-OHDA causes functional injury of renal proximal tubule cells, leading to impairment of transport processes across the cell membrane.