Affiliation:
1. Santen Pharmaceutical Co. Ltd, Nara Research and Development Center, Ophthalmic Research Division, 8916-16 Takayama-cho, Ikoma-shi 630-0101, Japan
Abstract
Abstract
Evidence for carrier-mediated transport of levofloxacin in the isolated rabbit cornea has been found. However, it is not known whether this mechanism is located in the epithelium or the endothelium. To resolve this question, we have measured the kinetics of levofloxacin uptake in primary cultures of rabbit corneal epithelial cells.
The results indicate that levofloxacin accumulation was time dependent and a steady state was reached after 30min. Maximal uptake occurred from a solution whose pH was 6.5. The uptake process was stereoselective and concentration dependent. In addition to the uptake, secretion of levofloxacin also occurred.
These results indicate that the corneal epithelium is the site of levofloxacin transport mechanisms, mediating both absorption and secretion.
Publisher
Oxford University Press (OUP)
Subject
Pharmaceutical Science,Pharmacology
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