An extract of Lannea microcarpa: composition, activity and evaluation of cutaneous irritation in cell cultures and reconstituted human epidermis

Author:

Picerno P1,Mencherini T1,Loggia R Delia2,Meloni M3,Sanogo R4,Aquino R P1

Affiliation:

1. School of Pharmacy and Master in Cosmetic Science and Technology, University of Salerno, Italy

2. Dipartimento di Economia e Merceologia, University of Trieste, Italy

3. VitroScreen Srl, Piazza Giulio Cesare 17, 20145, Milano, Italy

4. Faculte de Medicine, Farmacie et D'Odontostomatologie, University of Bamako, Mali

Abstract

Abstract Lannea microcarpa (Anacardiaceae) is a tropical tree used in African folk medicine and commercial dermopharmaceutical formulations. Fractionation and analysis of its polar extract allowed the identification of 4′-methoxy-myricetin 3-O-α-l-rhamnopyranoside, myricetin 3-O-α-l-rhamnopyranoside, myricetin 3-O-β-d-glucopyranoside, vitexin, isovitexin, gallic acid and epi-catechin, as the major constituents. In-vivo assay (the croton oil ear test in mice) showed that the extract had significant anti-inflammatory effect (ID50 = 900 μg cm2) but ten times lower than that of indometacin (ID50 = 93 μg cm2), the non-steroidal anti-inflammatory drug used as reference. Cytotoxicity and cutaneous irritation of the extract and its constituents were investigated. The crude extract and its major components did not affect cell viability in-vitro either in three different cultures (J774.A1, WEHI-164 and HEK-293) of cells grown in monolayers or in the reconstituted human epidermis (RHE, 3D model), nor did they cause release of pro-inflammatory mediators (IL-1α) or histomorphological modification of RHE.

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

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