Microscopic and Transcriptome Analysis Reveals that the Self-incompatibility in Rabbiteye Blueberry Belongs to the S-RNase-based Gametophytic Type

Author:

Yang Qin1,Fu Yan2,Liu Yalan1,Zhang Tingting1,Peng Shu1,Deng Jie1

Affiliation:

1. College of Life and Health Science, Kaili University, Kaili, Guizhou 556000, China; and Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou province, Kaili, Guizhou 556000, China

2. Qiandongnan National Polytechnic, Kaili 556000, China

Abstract

Berry fruits produced by Vaccinium (Ericaceae) plants are small but have a signature flavor and have become increasingly popular in the 21st century. However, self-incompatibility (SI) results in a relatively low fruit-set ratio and reduced fruit quality in Vaccinium. In this study, using Vaccinium ashei (V. ashei) styles after cross-pollination (CP) and self-pollination (SP) as material, transcriptomics and gene expression analyses were performed using high-throughput RNA sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). Subsequently, evolutionary analysis and conserved sequences analysis of candidate genes were conducted. Among the 135,324 unigenes, 30,863 were shown to be differentially expressed, and eight randomly selected differentially expressed genes were expressed in the styles at 96 hours after SP and CP. The transcriptomics and qRT-PCR results were significantly correlated, which confirmed the reliability of the differentially expressed genes obtained in our study. Compared with SP96, six differentially expressed ribonuclease T2 family genes were obtained in CP96, which were considered candidates for S-RNase. Additionally, the spatiotemporal and organizational expression trends of six candidates for S-RNase were confirmed by qRT-PCR, and the evolutionary and conservative sequence analysis indicated six candidate S-RNases with the typical S-RNase structure. The spatiotemporal and organizational expression results and evolutionary and conservative sequence analyses of the six candidate S-RNases suggest that SI in V. ashei is likely an S-RNase-mediated gametophytic one. This finding suggests the involvement of novel, previously undiscovered components involved in the V. ashei SI system. These findings help elucidate the molecular mechanisms of SI in rabbiteye blueberry and may also benefit breeding, production, and genomics research in V. ashei and other Vaccinium species.

Publisher

American Society for Horticultural Science

Reference64 articles.

1. Self-compatibility in peach [Prunus persica (L.) Batsch]: Patterns of diversity surrounding the S-locus and analysis of SFB alleles;Abdallah D,2020

2. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs;Altschul SF,1997

3. Differential expression analysis for sequence count data;Anders S,2010

4. UniProt: The universal protein knowledgebase;Apweiler R,2004

5. Gene ontology: Tool for the unification of biology. The Gene Ontology Consortium;Ashburner M,2000

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