Inheritance of the Anasazi Pattern of Partly Colored Seedcoats in Common Bean

Abstract

Inheritance of Anasazi pattern of partly colored seedcoats in common bean (Phaseolus vulgaris L.) was studied in a genetic stock t ana B V Anasazi BC3 5-593, whose Anasazi pattern is derived from Plant Introduction (PI) 451802. Line 5-593 is a determinate, Florida dry bean breeding line (with small black seeds) used as the recurrent parent in the development of many genetic stocks. The F2 from the cross t ana B V Anasazi BC3 5-593 × t z virgarcus BC3 5-593 segregated for two nonparental phenotypic classes and was consistent with the hypothesis that a single recessive gene, with tentative symbol ana, produces the Anasazi pattern with t Z ana and a new partly colored pattern Anabip with t z ana. Thus, the Anasazi factor is not an allele at the Z locus. Analysis of 57 random F3 progenies from the cross t ana B V Anasazi BC3 5-593 × t z virgarcus BC3 5-593 supported a genetic model where: 1) with t Z the Anasazi phenotype is controlled by a single recessive gene ana, i.e., genotype t Z ana, 2) the Anabip phenotype has the genotype t z ana, and 3) t Z/z ana produces a restricted Anasazi pattern. The allelism test cross t z ana Anabip BC3 5-593 × t z lers white BC3 5-593 produced complementation in the F2, demonstrating nonallelism of Ana (actually Bip) with the L locus. The allelism test cross t z ana Anabip BC3 5-593 × t z bip bipunctata BC3 5-593 failed to show complementation in F1 and F2, demonstrating allelism of Ana with the Bip locus. Using bulk segregant analysis, molecular markers linked in coupling to the Ana (OM9200, 5.4 cM) and Bip (OJ17700, 6.0 cM) genes were discovered. Allelism was also suggested by the result that the same linkage distance and recombination pattern were observed when the Ana marker was used to score the bipunctata population. We propose the gene symbol bipana for the recessive allele at the Bip locus that produces Anasazi pattern with genotype t Z bipana and the Anabip pattern with genotype t z bipana. Although bipana and bip are both recessive to Bip, their interactions with the Z locus are extraordinarily different. The pattern restrictive power of bipana expresses partly colored pattern with t Z, whereas bip requires t z to express partly colored pattern.