Regulated Water Deficits Improve Phytochemical Concentration in Lettuce

Abstract

In a growth chamber study, lettuce (Lactuca sativa) plants were used to evaluate the effects of water deficits on health-promoting phytochemicals with antioxidant properties. Lettuce plants were treated with water stress by withholding water once at 6 weeks after sowing for 2 days or multiple times at 4 weeks for 4 days, at 5 weeks for 3 days, and at 6 weeks for 2 days. Water stress increased the total phenolic concentration and antioxidant capacity in lettuce. Young seedlings, 7 days after germination, had the highest total phenolic concentration and antioxidant capacity, and also, younger plants were typically more responsive to water stress treatments in accumulating the antioxidants than older plants. Phenylalanine ammonia lyase and γ-tocopherol methyltransferase genes, involved in the biosynthesis of phenolic compounds and vitamin E, respectively, were activated in response to water stress, although no activation of L-galactose dehydrogenase was detected. Lettuce plants subjected to multiple water stress treatments accumulated significant amounts of chicoric acid compared with the control plants. Although the increase in antioxidant activity in water stress-treated plants at harvest was not as great as in young seedlings, it was significantly higher than the control. One-time water stress treatment of lettuce at the time of harvest did not result in any adverse effect on plant growth. Thus, these results show that mild water stress in lettuce applied just before harvest can enhance its crop quality with regard to its phytochemical concentration without any significant adverse effect on its growth or yield.