Thermotolerance in Lettuce Seeds: Association with Ethylene and Endo-β-mannanase

Abstract

Weakening of the endosperm tissue around the radicle tip before radicle protrusion and a potential role of endo-β-mannanase during germination of lettuce seeds (Lactuca sativa L.) at high temperature (35 °C) were investigated. Seeds from the thermotolerant genotypes `Everglades' and PI 251245 had greater endo-β-mannanase activity before radicle protrusion at 35 °C than the thermosensitive genotypes `Dark Green Boston', `Valmaine' and `Floricos 83'. Thermotolerant genotypes also generated more ethylene at high temperature. At 35 °C, germination of `Dark Green Boston' and `Everglades' seeds produced at days/nights of 20/10 °C was 10% and 32%, respectively, whereas germination of seeds produced at days/nights of 30/20 °C was 67% and 83%, respectively. Higher endo-β-mannanase activity was observed before radicle protrusion in `Dark Green Boston' seeds produced at 30/20 °C compared with those produced at 20/10 °C. A relationship between seed germination at high temperature, ethylene production, and an increase in endo-β-mannanase activity before radicle protrusion was confirmed.