Author:
Levi Amnon,Galau Glenn A.,Wetzstein Hazel Y.
Abstract
A simple and efficient protocol is reported for the isolation of RNA from embryos and leaves of pecan [Carya illinoinensis (Wangenh.) K. Koch]. The method relies on suppression of the polyphenols from interaction with the RNA and their rapid removal from the homogenate by chloroform extraction. This method produced abundant amounts of high-quality RNA. This protocol is likely to be useful for Juglandaceous species and other recalcitrant plants with high levels of phenolic compounds.
Publisher
American Society for Horticultural Science
Cited by
35 articles.
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