Micropropagation of Lowbush Blueberry from Mature Field-grown Plants

Abstract

Bud cultures from nonjuvenile field clones of lowbush blueberry (Vaccinium angustifolium Ait.) were established on Z-2 medium with 59 μm 2iP. Reversion to juvenile characteristics with small and rounder leaves occurred only on two explants after 19 weeks in culture. These shoots grew vigorously and could be easily subcultured. The number of shoots of one clone doubled every 23.3 days. Reducing the 2iP concentration to 12.3 and 24.6 μm reduced shoot proliferation, but permitted better shoot elongation. After 5 weeks in a mix of 3 peat: 2 vermiculite: 1 perlite, shoots >20 mm rooted better than shoots measuring 10 to 20 mm. Chemical names used: N-(3-methyl-2-butenyl)-1-H-purine-6-amine (2iP); 1H-indole-3-acetic acid (IAA).