Regeneration of Plants from Cell Suspensions of Lactuca saligna, Lactuca sativa, and Lactuca serriola

Abstract

Abstract ‘Black Seeded Simpson’, ‘Buttercrunch’, and ‘New York 12’ lettuce.(Lactuca sativa L.), Plant Introductions (PI) 261653 of Lactuca saligna L., and Acc. No. 500–4 of L. serriola L. regenerated plants from cell suspensions originated from leaf callus. Liquid cultures of these Lactuca spp. in either B5 or Murashige and Skoog (MS) basic medium amended with alpha-naphthaleneacetic acid (NAA) and 6-benzylamino purine (BA) developed roots, shoots, and complete plants when transferred to agar plates of B5 with several concentrations of BA under 16 hr of fluorescent illumination. Shoots were induced to produce a root system when cultured in B5 agar medium amended with NAA. Variation in response between cultivars and between species was observed, with L. serriola and ‘Black Seeded Simpson’ responding best to treatments.