Regeneration of Brassica oleracea from Peduncle Explants

Abstract

Peduncle explants from 12 Brassica oleracea L. lines representing five varieties [broccoli (italica), cabbage (capitata), cauliflower (botrytis), Chinese broccoli (alboglabra), and rapid-cycling B. oleracea] readily regenerated shoots in vitro. Average regeneration rates of more than 75% were obtained for most lines, with up to 35 shoots per explant. Shoots were visible within 7 to 10 days. Initial regeneration was polarized, occurring mainly from the basal end of explants. Linsmaier-Skoog-based medium containing 1 mg BA/liter was suitable for shoot regeneration from all 12 lines tested. Plants were rooted on hormone-free medium and transferred to soil. Chemical name used: benzyladenine (BA).