Vegetative Growth Responses of Citrus Nursery Trees to Various Growth Retardants

Abstract

As part of a larger study to improve rind color of citrus (Citrus spp.) fruit, this initial study was conducted to determine the concentration of various gibberellin-biosynthesis inhibitors required to elicit a biological response in citrus trees, as measured by vegetative growth. Paclobutrazol and GA3 were included as control treatments at concentrations known to elicit growth-retarding or growth-promoting effects, respectively. Repeated (×4) foliar applications of GA3 (at 64 ppm) increased growth of ‘Eureka’ lemon (Citrus limon) shoots by 63%, with no significant effect on rootstock and scion diameters. Repeated foliar applications of prohexadione-calcium (ProCa) at various concentrations (100, 200, 400, or 800 ppm) as well as uniconazole (at 500 or 1000 ppm) and paclobutrazol (at 0.25%) had no effect on rootstock or scion diameters 8 months after the first application. The high concentrations of ProCa (800 ppm) and uniconazole (1000 ppm), and the paclobutrazol treatment (0.25%) reduced shoot length compared with the control. Uniconazole at 1000 ppm resulted in the most growth retardation, which resulted in 34% shorter shoot length than the control. Although the number of nodes on the longest shoot did not differ from the untreated control, internode length differed significantly among treatments. ProCa at 400 and 800 ppm, uniconazole at 1000 ppm, and paclobutrazol at 0.25% significantly reduced internode length relative to the control by 31%, 56%, 50%, and 28%, respectively. Vegetative growth of ‘Eureka’ lemon nursery trees was retarded following the repeated (×4) foliar application of gibberellin-biosynthesis inhibitors. ProCa at 400 to 800 ppm and uniconazole at 1000 ppm were identified as prospective treatments for further field studies to test their effects on rind color enhancement of citrus fruit.