Differential Regulation of Genes Encoding Ethylene Biosynthesis Enzymes and Ethylene Response Sensor Ortholog during Ripening and in Response to Wounding in Avocados

Abstract

We investigated the differential regulation of two 1-aminocyclopropane-1-carboxylate synthase (ACS) genes, one 1-aminocyclopropane-1-carboxylate oxidase (ACO) gene and one ethylene response sensor (ERS1) ortholog during ripening and in response to wounding in avocados (Persea americana Mill. `Bacon'). The 1-aminocyclopropane-1-carboxylate (ACC) content, ACS activity and detectable expression of PA-ACS1 mRNA increased and reached a maximum prior to the climacteric peak, whereas ACO activity and the PA-ACO mRNA levels increased markedly only at the upsurge of ripening ethylene. A basal level of PA-ERS1 transcript was detected as from harvest, however, PA-ERS1 transcript was hyper-induced at the climacteric peak of ethylene production. 1-Methylcyclopropene (1-MCP) application at thepreclimacteric and the onset of climacteric stages inhibited the ACS and ACO activities, the transcription of PA-ACS1 and suppressed PA-ACO and PA-ERS1 mRNAs to trace levels. Discontinuation of 1-MCP treatment led to super-induction of PA-ACS1, PA-ACO, and PA-ERS1 transcripts. Wound induced ethylene biosynthesis and wound-induced PA-ACS2 mRNA accumulation were enhanced by 1-MCP, whereas wound-induced PA-ACO mRNA accumulation was unaffected by 1-MCP. These results indicate positive feedback regulation of the PA-ACS1 gene and negative feedback regulation of the PA-ACS2 gene by ethylene, while PA-ACO exhibits positive feedback regulation by ethylene and is also induced by wounding. The hyper-induction of PA-ERS1 mRNA at relatively high concentrations of ethylene may be a mechanism of avocados to regulate the ethylene responsiveness of the tissues by dissipation of the gas.