Influence of DB-cAMP, Adenosine, Forskolin, and Traumatic Acid on in Vitro Bulbing of Lilium longiflorum

Abstract

Both dibutyryl-cAMP and forskolin, with and without a pretreatment with traumatic acid, stimulated bulbing in vitro of Lilium longiflorum Thunb. by up to 65% over the controls. Dibutyryl-cAMP, forskolin, their combinations with traumatic acid, and adenosine significantly increased the micropropagation yield. Traumatic acid alone produced similar results on bulblet number per explant, but with increased fresh mass per bulblet. Chemical names used: 9-ß-D-ribofuranosyladenine (adenosine); adenosine 3′, 5′ - cyclic monophosphate (cAMP); dibutyryl adenosine 3′, 5′ - cyclic monophosphate (DB-cAMP); 9-ß-L (+) adenosine or 9-ß-L (+) ribofuranosyladenine; forskolin; 10(E) dodeca-1,12-dicarboxylic acid (traumatic acid).