A Simple, Room-temperature Extraction Method for 14Carbon-labeled Red Raspberry Leaf Tissues

Abstract

Labeled (`“C) compounds were recovered from tissue disks taken from 14CO2-fed leaves of l-year-old greenhouse-grown plants and l-month-old ex vitro transplants of red raspberry (Rubus idaeus L.) by hot (boiling in 80% ethanol immediately after 14C exposure), delayed-hot (boiling in 80% ethanol after a 2- to 3-day ethanol soak), and room-temperature (RT) (2-to 3-day soak in 80% ethanol) extraction methods. The RT extraction method was simple but as effective for extracting 14C-labeled compounds from red raspberry leaf tissues as hot and delayed-hot extraction methods.